Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis

Table 1 Primers sequences used to generate 7 PCR amplicons covering the variable regions in the bacterial 16S rRNA gene

Gene	Forward primer	Reverse primer
V1-V2	5′-TCGTCGGCAGCGTCAGAGTTTGATCCTGGCTCAG-3’	5′-GTCTCGTGGGCTCGGTGCTGCCTCCCGTAGGAGT-3’
V2	5′-TCGTCGGCAGCGTCAGYGGCGIACGGGTGAGTAA-3’	5′-GTCTCGTGGGCTCGGCYIACTGCTGCCTCCCGTAG-3’
V3_2	5′-TCGTCGGCAGCGTCCCTACGGGAGGCAGCAG-3’	5′-GTCTCGTGGGCTCGGGTATTACCGCGGCTGCTGG-3’
V4	5′-TCGTCGGCAGCGTCGTGCCAGCMGCCGCGGTAA-3’	5′-GTCTCGTGGGCTCGGGGACTACHVGGGTWTCTAAT-3’
V5-V6	5′-TCGTCGGCAGCGTCAGGATTAGATACCCTGGTA-3’	5′-GTCTCGTGGGCTCGGCRRCACGAGCTGACGAC-3’
V6_1	5′-TCGTCGGCAGCGTCAAACTCAAAKGAATTGACGG-3’	5′-GTCTCGTGGGCTCGGACGAGCTGACGACARCCATG-3’
V7-V8	5′-TCGTCGGCAGCGTCGYAACGAGCGCAACCC-3’	5′-GTCTCGTGGGCTCGGGACGGGCGGTGWGTRC-3’

ISSN: 2051-5960